Unique ID issued by UMIN | UMIN000009998 |
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Receipt number | R000011706 |
Scientific Title | Gene expression analaysis of the lateral spreading tumor |
Date of disclosure of the study information | 2013/02/08 |
Last modified on | 2015/08/08 09:56:16 |
Gene expression analaysis of the lateral spreading tumor
Gene expression analaysis of the lateral spreading tumor
Gene expression analaysis of the lateral spreading tumor
Gene expression analaysis of the lateral spreading tumor
Japan |
colorectal tumor
Gastroenterology |
Malignancy
NO
Decision of the therapeutic strategy and pridicting the prognosis of the LST by comparison of gene expression in LST with in colorectal tumor except LST.
Others
Gene expression analysis
Gene expression of targeted genes
Observational
20 | years-old | <= |
Not applicable |
Male and Female
Patients with colorectal tumor
Patients evaluated incompetent by doctors in attendance
200
1st name | |
Middle name | |
Last name | Makoto Arai |
Chiba University
Gastroenterology
1-8-1 Inohana, Chuo-ku, Chiba City
043-222-7171
araim-cib@umin.ac.jp
1st name | |
Middle name | |
Last name | Makoto Arai |
Chiba University
Gastroenterology
1-8-1 Inohana, Chuo-ku, Chiba City, JAPAN
043-226-2083
araim-cib@umin.ac.jp
Chiba University
Chiba University
Self funding
NO
2013 | Year | 02 | Month | 08 | Day |
Published
http://www.ncbi.nlm.nih.gov/pubmed/26048755
BACKGROUND:
Laterally spreading tumors (LSTs) are generally defined as lesions >10 mm in diameter, are characterized by lateral expansion along the luminal wall with a low vertical axis. In contrast to other forms of tumor, LSTs are generally considered to have a superficial growth pattern and the potential for malignancy. We focused on this morphological character of LSTs, and analyzed the gene expression profile of LSTs.
METHODS:
The expression of 168 genes in 41 colorectal tumor samples (17 LST-adenoma, 12 LST-carcinoma, 12 Ip [pedunculated type of the Paris classification)-adenoma, all of which were 10 mm or more in diameter] was analyzed by PCR array. Based on the results, we investigated the expression levels of genes up-regulated in LST-adenoma, compared to Ip-adenoma, by hierarchical and K-means clustering. To confirm the results of the array analysis, using an additional 60 samples (38 LST-adenoma, 22 Ip-adenoma), we determined the localization of the gene product by immunohistochemical staining.
RESULT:
The expression of 129 genes differed in colorectal tumors from normal mucosa by PCR array analysis. As a result of K-means clustering, the expression levels of five genes, AKT1, BCL2L1, ERBB2, MTA2 and TNFRSF25, were found to be significantly up-regulated (p < 0.05) in LST-adenoma, compared to Ip-adenoma. Immunohistochemical analysis showed that the BCL2L1 protein was significantly and meaningfully up-regulated in LST-adenoma compared to Ip-adenoma (p = 0.010). With respect to apoptosis status in LST-Adenoma, it assumes that BCL2L1 is anti-apoptotic protein, the samples such as BCL2L1 positive and TUNEL negative, or BCL2L1 negative and TUNEL positive are consistent with the assumption. 63.2 % LST-adenoma samples were consistent with the assumption.
CONCLUSIONS:
LSTs have an unusual profile of gene expression compared to other tumors and BCL2L1 might be concerned in the organization of LSTs.
Completed
2012 | Year | 01 | Month | 01 | Day |
2012 | Year | 01 | Month | 01 | Day |
Gene expression analaysis of colon LST
2013 | Year | 02 | Month | 08 | Day |
2015 | Year | 08 | Month | 08 | Day |
Value
https://center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000011706
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